glucose regulated protein 78 (Proteintech)
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glucose regulated protein 78
Glucose Regulated Protein 78, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+regulated+protein+78/GRP78%2CBIP+Fusion+Protein/pm41934679-85-66-80
Average 95 stars, based on 36 article reviews
Glucose Regulated Protein 78, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+regulated+protein+78/GRP78%2CBIP+Fusion+Protein/pm41934679-85-66-80
Average 95 stars, based on 36 article reviews
glucose regulated protein 78 - by Bioz Stars,
2026-09
95/100 stars
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Modification:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), H2O2 Assay:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Reverse Transcription:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Real-time Polymerase Chain Reaction:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Phospho-proteomics:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Fluorescence:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), Ubiquitin Proteomics:Article Title: <scp>ARIH1</scp> Promotes Preeclampsia by Inducing <scp>MFN2</scp>‐Dependent Hypoxia‐Triggered Mitophagy and Endoplasmic Reticulum Stress in Trophoblasts Article Snippet: The pathogenesis of preeclampsia (PE) involves endoplasmic reticulum stress (ERS) and the subsequent induction of mitophagy.. Ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1) is a key factor regulating mitophagy, but its role in PE has not been reported.. In this study, we aimed to analyze the role of ARIH1 in the pathogenesis of PE. Membrane:Article Title: <scp>ARIH1</scp> Promotes Preeclampsia by Inducing <scp>MFN2</scp>‐Dependent Hypoxia‐Triggered Mitophagy and Endoplasmic Reticulum Stress in Trophoblasts Article Snippet: The pathogenesis of preeclampsia (PE) involves endoplasmic reticulum stress (ERS) and the subsequent induction of mitophagy.. Ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1) is a key factor regulating mitophagy, but its role in PE has not been reported.. In this study, we aimed to analyze the role of ARIH1 in the pathogenesis of PE. Polymerase Chain Reaction:Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts. Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), other:Article Title: BFGF downregulates endoplasmic reticulum stress to alleviate HUVEC cell damage under high-glucose and inflammatory conditions. Article Snippet: Primary antibodies to proliferating cell nuclear antigen (PCNA, 60097-1-Ig, 1:1000), B-cell lymphoma-2 (Bcl-2, 68103-1-Ig, 1:1000), Bcl-2-associated x (Bax, 50599-2-Ig, 1:1000), cleaved-caspase 3 (68773-1-Ig, 1:1000), activating transcription factor 4 (ATF4, 10835-1-AP, 1:1000), phospho-eukaryotic translation initiation factor 2 (p-eIF2, 68023-1-Ig, 1:1000), glucose-regulated protein 78 (GRP78, 66574-1-Ig, 1:1000), C/EBP-homologous protein (CHOP, 66741-1-Ig, 1:1000), and protein kinase RNA-like ER kinase (PERK, 68482-1-Ig, 1:1000) were purchased from the Binding Assay:Article Title: Nrf2 maintains reactive oxygen species at an optimal level during endoplasmic reticulum stress in HT22 cells. Article Snippet: Protein concentrations were measured using the DC Protein Assay kit (Bio-Rad Laboratories), and equal amounts of protein (20 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Buckinghamshire, UK). .. Membranes were probed with the following primary antibodies: HO-1 (Proteintech, Rosemont, IL, USA, RRID:AB_2882091); GCLC (Santa Cruz Biotechnology, RRID: AB_2736837); peroxiredoxin 4 (PRDX4; Proteintech, RRID: AB_2881403); growth arrest and DNA damage-inducible gene 153 (GADD153; Santa Cruz Biotechnology, RRID:AB_627411); spliced X-box binding protein 1 (sXBP1; Abcam, Cambridge, UK, Cat# ab220783); KDEL (MBL, Tokyo, Japan, RRID:AB_10693914), which detects KDEL-containing proteins such as glucose-regulated protein 94 (GRP94; 94 kDa), |